• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PMS2 Recombinant antibody (84894-5-RR)

    WB analysis using 84894-5-RR (same clone as 84894-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84894-5-RR (PMS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PMS2 Recombinant antibody (84894-5-RR)

    IHC staining of human colon cancer using 84894-5-RR (same clone as 84894-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84894-5-RR (PMS2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PMS2 Recombinant antibody (84894-5-RR)

    IHC staining of human colon cancer using 84894-5-RR (same clone as 84894-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84894-5-RR (PMS2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using PMS2 Recombinant antibody (84894-5-RR)

    FC experiment of MCF-7 using 84894-5-RR (same clone as 84894-5-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PMS2 Recombinant antibody (84894-5-RR, Clone:242455F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84894-5-RR

    Affinity and Kinetic Characterization of 84894-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84894-5-RR against Human PMS2 were performed. The affinity constant is below 1 pM.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

PMS2 Recombinant antibody, PBS Only

PMS2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 84894-5-PBS
Clone No.242455F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

PMSL2, PMS2CL, PMS1 protein homolog 2, HNPCC4, EC:3.1.-.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84894-5-PBS targets PMS2 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PMS2 fusion protein Ag33508 相同性解析による交差性が予測される生物種
Full Name PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
Calculated molecular weight 862 aa, 96 kDa
Observed molecular weight117 kDa
GenBank accession numberBC093921
Gene Symbol PMS2
Gene ID (NCBI) 5395
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP54278
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS).