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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PNMT Polyclonal antibody

PNMT Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 13217-1-AP

Synonyms

PNMTase, Phenylethanolamine N-methyltransferase, PENT, Noradrenaline N-methyltransferase, EC:2.1.1.28

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of K-562 cells using PNMT Polyclonal antibody (13217-1-AP)

    WB analysis of K-562 using 13217-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13217-1-AP (PNMT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-12 cells using PNMT Polyclonal antibody (13217-1-AP)

    IP experiment of PC-12 using 13217-1-AP

    IP result of anti-PNMT (IP:13217-1-AP, 4ug; Detection:13217-1-AP 1:300) with PC-12 cells lysate 2400ug.

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Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13217-1-AP targets PNMT in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PNMT fusion protein Ag4098 相同性解析による交差性が予測される生物種
Full Name phenylethanolamine N-methyltransferase
Calculated molecular weight 282 aa, 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC037246
Gene symbol PNMT
Gene ID (NCBI) 5409
RRIDAB_2167781
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phenylethanolamine N-methyltransferase(PNMT), the final enzyme in catecholamine biosynthesis, converts norepinephrine to epinephrine, thereby serving as a marker of the adrenergic phenotype.

Protocols

Product Specific Protocols
WB protocol for PNMT antibody 13217-1-APDownload protocol
IP protocol for PNMT antibody 13217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Intermittent Hypoxia Increased the Expression of DBH and PNMT in Neuroblastoma Cells via MicroRNA-375-Mediated Mechanism.

Authors - Shin Takasawa
View Article
humanWB

Front Endocrinol (Lausanne)

Clinical identification of expressed proteins in adrenal medullary hyperplasia detected with hypertension

Authors - He Ma
View Article