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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PNO1 Polyclonal antibody (21059-1-AP)

    WB analysis using 21059-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21059-1-AP (PNO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PNO1 Polyclonal antibody (21059-1-AP)

    WB analysis of K-562 using 21059-1-AP

    WB result of PNO1 antibody (21059-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-PNO1 transfected K-562 cells.

  • Western Blot (WB) analysis of mouse spleen tissue using PNO1 Polyclonal antibody (21059-1-AP)

    WB analysis of mouse spleen using 21059-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21059-1-AP (PNO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of L02 cells using PNO1 Polyclonal antibody (21059-1-AP)

    IP experiment of L02 using 21059-1-AP

    IP result of anti-PNO1 (IP:21059-1-AP, 3ug; Detection:21059-1-AP 1:300) with L02 cells lysate 1500ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PNO1 Polyclonal antibody (21059-1-AP)

    IHC staining of human liver cancer using 21059-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21059-1-AP (PNO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using PNO1 Polyclonal antibody (21059-1-AP)

    IHC staining of human urothelial carcinoma using 21059-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21059-1-AP (PNO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using PNO1 Polyclonal antibody (21059-1-AP)

    IHC staining of human urothelial carcinoma using 21059-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21059-1-AP (PNO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

PNO1 Polyclonal antibody

PNO1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 21059-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

KHRBP1, PNO1, RNA binding protein PNO1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA2780 cells, mouse spleen tissue, K-562 cells, A549 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman liver cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21059-1-AP targets PNO1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PNO1 fusion protein Ag14752 相同性解析による交差性が予測される生物種
Full Name partner of NOB1 homolog (S. cerevisiae)
Calculated molecular weight 252 aa, 28 kDa
Observed molecular weight 28-35 kDa
GenBank accession numberBC008304
Gene Symbol PNO1
Gene ID (NCBI) 56902
RRIDAB_10734582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRX1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The function of PNO1 remains largely unknown in mammal. PNO1 plays a role in proteasome and ribosome neogenesis in yeast.

Protocols

Product Specific Protocols
WB protocol for PNO1 antibody 21059-1-APDownload protocol
IHC protocol for PNO1 antibody 21059-1-APDownload protocol
IP protocol for PNO1 antibody 21059-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

PNO1 promotes the progression of osteosarcoma via TGF-β and YAP/TAZ pathway

Authors - Long Fang
  • KD Validated
View Article
humanIHC

Heliyon

Pan-cancer exploration of PNO1: A prospective prognostic biomarker with ties to immune infiltration

Authors - Yinhui Qin
View Article