PNPLA3 Polyclonal antibody

PNPLA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11442-1-AP

Synonyms

Acylglycerol O acyltransferase, Adiponutrin, ADPN, C22orf20, iPLA(2)epsilon, iPLA2 epsilon, PNPLA3


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Tested Applications

Positive WB detected inL02 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

11442-1-AP targets PNPLA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PNPLA3 fusion protein Ag1959 相同性解析による交差性が予測される生物種
Full Name patatin-like phospholipase domain containing 3
Calculated molecular weight 481 aa, 53 kDa
Observed molecular weight 50-70 kDa, 130-150 kDa
GenBank accession numberBC014449
Gene symbol PNPLA3
Gene ID (NCBI) 80339
RRIDAB_2918026
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PNPLA3 belongs to a family of proteins that share a domain that was first identified in patatin, a major soluble protein of potato tubers with nonspecific acyl hydrolase activity. The domain differs from classical lipases by employing a catalytic dyad (Ser-Asp), rather than a catalytic triad, to effect hydrolysis. PNPLA3 is expressed primarily in liver and adipose tissue, in which it partitions to membranes and lipid droplets. Real-time PCR of cDNA from human tissues indicated that PNPLA3 expression was highest in the liver, followed by skin and adipose tissue. PNPLA3 is expressed at the highest levels in adipose tissue of mice. The PNPLA3 protein was expected at 50-70 kDa, as observed; the additional band at approximately 130-150 kDa is a oligomer bands. (PMID: 20385813, PMID: 24931521, PMID: 23023705)

Protocols

Product Specific Protocols
WB protocol for PNPLA3 antibody 11442-1-APDownload protocol
IHC protocol for PNPLA3 antibody 11442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Diabetes

Rab8a Deficiency in Skeletal Muscle Causes Hyperlipidemia and Hepatosteatosis Via Impairment of Muscle Lipid Uptake and Storage.

Authors - Qiaoli Chen
human,ratWB

Arch Biochem Biophys

Baicalin ameliorates alcohol-induced hepatic steatosis by suppressing SREBP1c elicited PNPLA3 competitive binding to ATGL.

Authors - Pan Li
humanWB

Diabetes

N-end Rule-Mediated Proteasomal Degradation of ATGL Promotes Lipid Storage

Authors - Jiesi Xu
zebrafishWB

Gene

Investigation on the mechanism of hepatotoxicity of dictamnine on juvenile zebrafish by integrating metabolomics and transcriptomics

Authors - Xu Xiaomin