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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PNPLA6 Polyclonal antibody

PNPLA6 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14261-1-AP

Synonyms

sws, SPG39, Patatin-like phospholipase domain-containing protein 6, NTEMND, NTE

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using PNPLA6 Polyclonal antibody (14261-1-AP)

    WB analysis of HEK-293 using 14261-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14261-1-AP (PNPLA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using PNPLA6 Polyclonal antibody (14261-1-AP)

    WB analysis of SH-SY5Y using 14261-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14261-1-AP (PNPLA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment using PNPLA6 Polyclonal antibody (14261-1-AP)

    IP experiment using 14261-1-AP

    IP result of anti-PNPLA6 (14261-1-AP for IP and Detection) with HEK-293 cells.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PNPLA6 Polyclonal antibody (14261-1-AP)

    IF Staining of HepG2 using 14261-1-AP

    Immunofluorescent analysis of HepG2 cells, using PNPLA6 antibody 14261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PNPLA6 Polyclonal antibody (14261-1-AP)

    IF Staining of MCF-7 using 14261-1-AP

    Immunofluorescent analysis of MCF-7 cells, using PNPLA6 antibody 14261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inHEK-293 cells, SH-SY5Y cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14261-1-AP targets PNPLA6 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PNPLA6 fusion protein Ag5546 相同性解析による交差性が予測される生物種
Full Name patatin-like phospholipase domain containing 6
Calculated molecular weight 150 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC051768
Gene symbol PNPLA6
Gene ID (NCBI) 10908
RRIDAB_2283965
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PNPLA6(patatin-like phospholipase domain-containing protein 6) is also named as NTE(neuropathy target esterase) and belongs to the NTE family. It plays an important role in motor neuron development and implicated overexpression of BMP signaling(PMID:22996643). Defects in PNPLA6 are the cause of spastic paraplegia autosomal recessive type 39 (SPG39)(PMID:18313024). This protein has a glycosylaion site that it can be glycosylated(PMID:9576844). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PNPLA6 antibody 14261-1-APDownload protocol
IF protocol for PNPLA6 antibody 14261-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

FMRP targets distinct mRNA sequence elements to regulate protein expression.

Authors - Ascano Manuel M Jr
View Article

Mol Biol Cell

Lowe syndrome-linked endocytic adaptors direct membrane cycling kinetics with OCRL in Dictyostelium discoideum.

Authors - Alexandre Luscher
View Article