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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PNPO Polyclonal antibody

PNPO Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15552-1-AP

Synonyms

Pyridoxine-5'-phosphate oxidase, Pyridoxine 5 phosphate oxidase, Pyridoxamine-phosphate oxidase, Pyridoxamine phosphate oxidase, PDXPO

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PNPO Polyclonal antibody (15552-1-AP)

    WB analysis using 15552-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using PNPO Polyclonal antibody (15552-1-AP)

    WB analysis of human heart using 15552-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PNPO Polyclonal antibody (15552-1-AP)

    WB analysis of human brain using 15552-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using PNPO Polyclonal antibody (15552-1-AP)

    WB analysis of human liver using 15552-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using PNPO Polyclonal antibody (15552-1-AP)

    IP experiment of MCF-7 using 15552-1-AP

    IP result of anti-PNPO (IP:15552-1-AP, 4ug; Detection:15552-1-AP 1:500) with MCF-7 cells lysate 3200 ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using PNPO Polyclonal antibody (15552-1-AP)

    IHC staining of human liver using 15552-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15552-1-AP (PNPO antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PNPO Polyclonal antibody (15552-1-AP)

    IHC staining of human liver using 15552-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15552-1-AP (PNPO antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PNPO Polyclonal antibody (15552-1-AP)

    IF Staining of HeLa using 15552-1-AP

    Immunofluorescent analysis of Hela cells, using PNPO antibody 15552-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inMCF-7 cells, human brain tissue, human heart tissue, human liver tissue, mouse brain tissue, rat brain tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15552-1-AP targets PNPO in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PNPO fusion protein Ag7914 相同性解析による交差性が予測される生物種
Full Name pyridoxamine 5'-phosphate oxidase
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC006525
Gene symbol PNPO
Gene ID (NCBI) 55163
RRIDAB_2165814
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PNPO antibody 15552-1-APDownload protocol
IHC protocol for PNPO antibody 15552-1-APDownload protocol
IF protocol for PNPO antibody 15552-1-APDownload protocol
IP protocol for PNPO antibody 15552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Cell Dev Biol

Prognostic Implication and Oncogenic Role of PNPO in Pan-Cancer.

Authors - Lingyun Zhang
View Article
WB

bioRxiv

Patient mutations in DRP1 perturb synaptic maturation of cortical neurons

Authors - T B Baum
View Article
mouseIHC

Apoptosis

Targeting PNPO to suppress tumor growth via inhibiting autophagic flux and to reverse paclitaxel resistance in ovarian cancer

Authors - Xin Li
View Article
mouseWB

Nat Metab

PNPO-PLP axis senses prolonged hypoxia in macrophages by regulating lysosomal activity

Authors - Hiroki Sekine
View Article