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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PODXL2 Polyclonal antibody

PODXL2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16383-1-AP

Synonyms

Endoglycan, PODLX2, podocalyxin like 2, Podocalyxin like protein 2, PODXL2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using PODXL2 Polyclonal antibody (16383-1-AP)

    WB analysis of mouse pancreas using 16383-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16383-1-AP (PODXL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using PODXL2 Polyclonal antibody (16383-1-AP)

    IP experiment of K-562 using 16383-1-AP

    IP result of anti-PODXL2 (IP:16383-1-AP, 4ug; Detection:16383-1-AP 1:300) with K-562 cells lysate 4000ug.

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Tested Applications

Positive WB detected inmouse pancreas tissue
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16383-1-AP targets PODXL2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PODXL2 fusion protein Ag9374 相同性解析による交差性が予測される生物種
Full Name podocalyxin-like 2
Calculated molecular weight605aa,65 kDa; 529aa,57 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC019330
Gene symbol PODXL2
Gene ID (NCBI) 50512
RRIDAB_2166112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PODXL2 antibody 16383-1-APDownload protocol
IP protocol for PODXL2 antibody 16383-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Med

Vascular niche E-selectin regulates hematopoietic stem cell dormancy, self renewal and chemoresistance.

Authors - Ingrid G Winkler
View Article