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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POF1B Polyclonal antibody

POF1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11398-1-AP

Synonyms

Protein POF1B, premature ovarian failure, 1B, POF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POF1B Polyclonal antibody (11398-1-AP)

    WB analysis using 11398-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11398-1-AP (POF1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using POF1B Polyclonal antibody (11398-1-AP)

    IHC staining of human stomach cancer using 11398-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11398-1-AP (POF1B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using POF1B Polyclonal antibody (11398-1-AP)

    IHC staining of human ovary cancer using 11398-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11398-1-AP (POF1B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using POF1B Polyclonal antibody (11398-1-AP)

    IF Staining of MCF-7 using 11398-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 11398-1-AP (POF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, mouse colon tissue
Positive IHC detected inhuman ovary cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11398-1-AP targets POF1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POF1B fusion protein Ag1945 相同性解析による交差性が予測される生物種
Full Name premature ovarian failure, 1B
Calculated molecular weight 595 aa, 69 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC017500
Gene symbol POF1B
Gene ID (NCBI) 79983
RRIDAB_2252552
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POF1B plays a key role in the organization of epithelial monolayers by regulating the actin cytoskeleton. POF1B is a candidate gene for premature ovarian failure (POF); it is mainly expressed in polarised epithelial tissues.

Protocols

Product Specific Protocols
WB protocol for POF1B antibody 11398-1-APDownload protocol
IHC protocol for POF1B antibody 11398-1-APDownload protocol
IF protocol for POF1B antibody 11398-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols