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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using POGK Polyclonal antibody (24888-1-AP)

    WB analysis of A549 using 24888-1-AP (same clone as 24888-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 24888-1-AP (POGK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24888-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using POGK Polyclonal antibody (24888-1-AP)

    WB analysis of MCF-7 using 24888-1-AP (same clone as 24888-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24888-1-AP (POGK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24888-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using POGK Polyclonal antibody (24888-1-AP)

    WB analysis of human testis using 24888-1-AP (same clone as 24888-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 24888-1-AP (POGK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24888-1-PBS in a different storage buffer formulation.

POGK Polyclonal antibody, PBS Only

POGK Polyclonal Antibody for WB, Indirect ELISA
Cat No. 24888-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24888-1-PBS targets POGK in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21681

Product name: Recombinant human POGK protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 260-609 aa of BC131624

Sequence: ALVDQRVAEYVRYMQAKGDPITREAMQLKALEIAQEMNIPEKGFKASLGWCRRMMRRYDLSLRHKVPVPQHLPEDLTEKLVTYQRSVLALRRAHDYEVAQMGNADETPICLEVPSRVTVDNQGEKPVLVKTPGREKLKITAMLGVLADGRKLPPYIILRGTYIPPGKFPSGMEIRCHRYGWMTEDLMQDWLEVVWRRRTGAVPKQRGMLILNGFRGHATDSVKNSMESMNTDMVIIPGGLTSQLQVLDVVVYKPLNDSVRAQYSNWLLAGNLALSPTGNAKKPPLGLFLEWVMVAWNSISSESIVQGFKKCHISSNLEEEDDVLWEIESELPGGGEPPKDCDTESMAESN

相同性解析による交差性が予測される生物種
Full Name pogo transposable element with KRAB domain
Calculated molecular weight 609 aa, 69 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC131624
Gene Symbol POGK
Gene ID (NCBI) 57645
RRIDAB_2879779
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P215
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

POGK also named as pogo transposable element with KRAB domain is a 609 amino acid protein, which contains one DDE domain, 1 KRAB domain and one HTH CENPB-type DNA binding domain. POGK belongs to DNA-mediated transposons family and may involve in the regulation of gene transcription.

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