• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POGZ Polyclonal antibody, PBS Only

POGZ Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30106-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using POGZ Polyclonal antibody (30106-1-AP)

    WB analysis using 30106-1-AP (same clone as 30106-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30106-1-AP (POGZ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using POGZ Polyclonal antibody (30106-1-AP)

    IP experiment of HeLa using 30106-1-AP (same clone as 30106-1-PBS)

    IP result of anti-POGZ (IP:30106-1-AP, 4ug; Detection:30106-1-AP 1:2000) with HeLa cells lysate 1800 ug. This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using POGZ Polyclonal antibody (30106-1-AP)

    IHC staining of mouse brain using 30106-1-AP (same clone as 30106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using POGZ Polyclonal antibody (30106-1-AP)

    IHC staining of mouse testis using 30106-1-AP (same clone as 30106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using POGZ Polyclonal antibody (30106-1-AP)

    IHC staining of mouse testis using 30106-1-AP (same clone as 30106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using POGZ Polyclonal antibody (30106-1-AP)

    IHC staining of mouse brain using 30106-1-AP (same clone as 30106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30106-1-AP (POGZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POGZ Polyclonal antibody (30106-1-AP)

    IF Staining of HepG2 using 30106-1-AP (same clone as 30106-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POGZ antibody (30106-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using POGZ Polyclonal antibody (30106-1-AP)

    IF Staining of U-251 using 30106-1-AP (same clone as 30106-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using POGZ antibody (30106-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30106-1-PBS in a different storage buffer formulation.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30106-1-PBS targets POGZ in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POGZ fusion protein Ag32435 相同性解析による交差性が予測される生物種
Full Name pogo transposable element with ZNF domain
Calculated molecular weight155kd
Observed molecular weight 155 kDa
GenBank accession numberNM_015100
Gene symbol POGZ
Gene ID (NCBI) 23126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

POGZ, also known as MRD37, is a zinc-finger protein containing 9 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family. POGZ plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. POGZ plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms (PMID:20562864). Heterozygous mutation of POGZ causes White-Sutton syndrome (PMID: 26739615). Expect a band approximately 155 kDa in size corresponding to POGZ by western blotting.