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DNA Polymerase Beta Polyclonal antibody
DNA Polymerase Beta Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 18003-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | COLO 320 cells, K-562 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human testis tissue, human brain tissue, human kidney tissue, human ovary tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 7 publications below |
Product Information
18003-1-AP targets DNA Polymerase Beta in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, rat, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DNA Polymerase Beta fusion protein Ag12496 相同性解析による交差性が予測される生物種 |
Full Name | polymerase (DNA directed), beta |
Calculated molecular weight | 335 aa, 38 kDa |
Observed molecular weight | 38 kDa |
GenBank accession number | BC100288 |
Gene symbol | DNA Polymerase Beta |
Gene ID (NCBI) | 5423 |
RRID | AB_2299845 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for DNA Polymerase Beta antibody 18003-1-AP | Download protocol |
IHC protocol for DNA Polymerase Beta antibody 18003-1-AP | Download protocol |
IP protocol for DNA Polymerase Beta antibody 18003-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Adv Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair | ||
Nat Commun Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia. | ||
Proc Natl Acad Sci U S A Ligand-induced gene activation is associated with oxidative genome damage whose repair is required for transcription. | ||
Cancer Cell Int Overexpression of A613T and G462T variants of DNA polymerase β weakens chemotherapy sensitivity in esophageal cancer cell lines.
| ||
DNA Repair (Amst) Acetylation of oxidized base repair-initiating NEIL1 DNA glycosylase required for chromatin-bound repair complex formation in the human genome increases cellular resistance to oxidative stress. | ||
NAR Cancer XRCC1 promotes replication restart, nascent fork degradation and mutagenic DNA repair in BRCA2-deficient cells. |