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KD/KO Validated

DNA Polymerase Beta Polyclonal antibody

Name: DNA Polymerase Beta Polyclonal antibody
Brand: Proteintech
SKU: 18003-1-AP

DNA Polymerase Beta Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18003-1-AP

Synonyms

DNA polymerase beta, POLB

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis using 18003-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 18003-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 18003-1-AP
IP result of anti-DNA Polymerase Beta (IP:18003-1-AP, 4ug; Detection:18003-1-AP 1:800) with K-562 cells lysate 3200ug.
IHC staining of human testis using 18003-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 18003-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 18003-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 18003-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 18003-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 18003-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 18003-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 18003-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 18003-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 18003-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

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Cat No. 18003-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	COLO 320 cells, K-562 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human testis tissue, human brain tissue, human kidney tissue, human ovary tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below

Product Information

18003-1-AP targets DNA Polymerase Beta in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DNA Polymerase Beta fusion protein Ag12496 相同性解析による交差性が予測される生物種
Full Name	polymerase (DNA directed), beta
Calculated molecular weight	335 aa, 38 kDa
Observed molecular weight	38 kDa
GenBank accession number	BC100288
Gene symbol	DNA Polymerase Beta
Gene ID (NCBI)	5423
RRID	AB_2299845
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for DNA Polymerase Beta antibody 18003-1-AP	Download protocol
IHC protocol for DNA Polymerase Beta antibody 18003-1-AP	Download protocol
IP protocol for DNA Polymerase Beta antibody 18003-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Adv Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair Authors - Dinh Duc Nguyen View Article
human	WB	Nat Commun Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia. Authors - Hu Lei View Article
human		Proc Natl Acad Sci U S A Ligand-induced gene activation is associated with oxidative genome damage whose repair is required for transcription. Authors - Shiladitya Sengupta View Article
mouse	WB	Cancer Cell Int Overexpression of A613T and G462T variants of DNA polymerase β weakens chemotherapy sensitivity in esophageal cancer cell lines. Authors - Yuanyuan Wang KO Validated View Article
human	WB	DNA Repair (Amst) Acetylation of oxidized base repair-initiating NEIL1 DNA glycosylase required for chromatin-bound repair complex formation in the human genome increases cellular resistance to oxidative stress. Authors - Shiladitya Sengupta View Article
human		NAR Cancer XRCC1 promotes replication restart, nascent fork degradation and mutagenic DNA repair in BRCA2-deficient cells. Authors - Bradley J Eckelmann View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNA Polymerase Beta Polyclonal antibody

DNA Polymerase Beta Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 18003-1-AP

WB analysis of K-562 using 18003-1-AP

IP experiment of K-562 using 18003-1-AP

IHC staining of human testis using 18003-1-AP

IHC staining of human testis using 18003-1-AP

IHC staining of human brain using 18003-1-AP

IHC staining of human brain using 18003-1-AP

IHC staining of human spleen using 18003-1-AP

IHC staining of human spleen using 18003-1-AP

IHC staining of human ovary using 18003-1-AP

IHC staining of human kidney using 18003-1-AP

IHC staining of human kidney using 18003-1-AP

IHC staining of human ovary using 18003-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair

Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.

Ligand-induced gene activation is associated with oxidative genome damage whose repair is required for transcription.

Overexpression of A613T and G462T variants of DNA polymerase β weakens chemotherapy sensitivity in esophageal cancer cell lines.

Acetylation of oxidized base repair-initiating NEIL1 DNA glycosylase required for chromatin-bound repair complex formation in the human genome increases cellular resistance to oxidative stress.

XRCC1 promotes replication restart, nascent fork degradation and mutagenic DNA repair in BRCA2-deficient cells.