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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLD1 Polyclonal antibody (15646-1-AP)

    WB analysis using 15646-1-AP (same clone as 15646-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15646-1-AP (POLD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using POLD1 Polyclonal antibody (15646-1-AP)

    IP experiment of HeLa using 15646-1-AP (same clone as 15646-1-PBS)

    IP result of anti-POLD1 (IP:15646-1-AP, 4ug; Detection:15646-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using POLD1 Polyclonal antibody (15646-1-AP)

    IHC staining of human breast cancer using 15646-1-AP (same clone as 15646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using POLD1 Polyclonal antibody (15646-1-AP)

    IHC staining of human breast cancer using 15646-1-AP (same clone as 15646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using POLD1 Polyclonal antibody (15646-1-AP)

    IHC staining of human colon cancer using 15646-1-AP (same clone as 15646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using POLD1 Polyclonal antibody (15646-1-AP)

    IHC staining of human colon cancer using 15646-1-AP (same clone as 15646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

POLD1 Polyclonal antibody, PBS Only

POLD1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15646-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15646-1-PBS targets POLD1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLD1 fusion protein Ag8178 相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), delta 1, catalytic subunit 125kDa
Calculated molecular weight 1107 aa, 124 kDa
Observed molecular weight 124 kDa
GenBank accession numberBC008800
Gene Symbol POLD1
Gene ID (NCBI) 5424
RRIDAB_2252572
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28340
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

POLD1, also known as DNA polymerase subunit delta p125, is 1107 amino acid protein, which belongs to the DNA polymerase type-B family. POLD1 colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. A previous study demonstrated that overexpression of POLD1 is associated with an increased risk of breast cancer. (PMID: 30344713 )