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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POLD2 Polyclonal antibody

POLD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29765-1-AP

Synonyms

DNA polymerase delta subunit 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLD2 Polyclonal antibody (29765-1-AP)

    WB analysis using 29765-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using POLD2 Polyclonal antibody (29765-1-AP)

    WB analysis using 29765-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using POLD2 Polyclonal antibody (29765-1-AP)

    WB analysis using 29765-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using POLD2 Polyclonal antibody (29765-1-AP)

    IHC staining of human colon using 29765-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using POLD2 Polyclonal antibody (29765-1-AP)

    IHC staining of human colon using 29765-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using POLD2 Polyclonal antibody (29765-1-AP)

    IHC staining of human lung cancer using 29765-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using POLD2 Polyclonal antibody (29765-1-AP)

    IHC staining of human lung cancer using 29765-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POLD2 Polyclonal antibody (29765-1-AP)

    IF Staining of HeLa using 29765-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLD2 antibody (29765-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293T cells, NIH/3T3 cells, HeLa cells, Jurkat cells, SGC-7901 cells, U-251 cells, mouse colon tissue, mouse pancreas tissue, rat colon tissue
Positive IHC detected inhuman colon tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29765-1-AP targets POLD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLD2 fusion protein Ag31521 相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), delta 2, regulatory subunit 50kDa
Calculated molecular weight 51 kDa
Observed molecular weight48-51 kDa
GenBank accession numberBC000459
Gene symbol POLD2
Gene ID (NCBI) 5425
RRIDAB_2918344
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POLD2, also named as DNA polymerase delta subunit 2, is a 469 amino acid protein, which belongs to the DNA polymerase delta/II small subunit family. The function of the small subunit is not yet clear. POLD2 has an essential function for the small subunit in the heterodimeric core enzyme.

Protocols

Product Specific Protocols
WB protocol for POLD2 antibody 29765-1-APDownload protocol
IHC protocol for POLD2 antibody 29765-1-APDownload protocol
IF protocol for POLD2 antibody 29765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols