POLDIP2 Polyclonal antibody
POLDIP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15080-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
15080-1-AP targets POLDIP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | POLDIP2 fusion protein Ag7154 相同性解析による交差性が予測される生物種 |
| Full Name | polymerase (DNA-directed), delta interacting protein 2 |
| Calculated molecular weight | 42 kDa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | BC000655 |
| Gene symbol | POLDIP2 |
| Gene ID (NCBI) | 26073 |
| RRID | AB_2166599 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
POLDIP2(Polymerase delta-interacting protein 2) is also named as PDIP38, POLD4. It is as a unique positive regulator of Nox4 via its association with p22phox and together with Nox4, has profound effects on Rhodependent cytoskeletal remodeling. It is also as a regulator of cell division. It consists of 368 amino acids and has a predicted molecular mass of 42 kDa, with a potential signal peptide cleavage site after the first N-terminal 48 residues, which would result in a protein of 37 kDa(PMID:19574552). Endogenous human and rat POLDIP2 localized to the nucleus, plasma membrane, and cytoplasm(PMID:17623671).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POLDIP2 antibody 15080-1-AP | Download protocol |
| IHC protocol for POLDIP2 antibody 15080-1-AP | Download protocol |
| IF protocol for POLDIP2 antibody 15080-1-AP | Download protocol |
| IP protocol for POLDIP2 antibody 15080-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A DNA polymerase δ-interacting protein 2 is a processivity factor for DNA polymerase λ during 8-oxo-7,8-dihydroguanine bypass. | ||
Cell Cycle PDIP38 is translocated to the spliceosomes/nuclear speckles in response to UV-induced DNA damage and is required for UV-induced alternative splicing of MDM2. | ||
Cells Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA. |
