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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SKAR Polyclonal antibody

SKAR Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17466-1-AP

Synonyms

KIAA1649, p46, PDIP46, POLDIP3, S6K1 Aly/REF like target, SKAR

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SKAR Polyclonal antibody (17466-1-AP)

    WB analysis of HeLa using 17466-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SKAR Polyclonal antibody (17466-1-AP)

    WB analysis of A549 using 17466-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SKAR Polyclonal antibody (17466-1-AP)

    WB analysis of HepG2 using 17466-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SKAR Polyclonal antibody (17466-1-AP)

    WB analysis of mouse brain using 17466-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SKAR Polyclonal antibody (17466-1-AP)

    IP experiment of mouse brain using 17466-1-AP

    IP result of anti-SKAR (IP:17466-1-AP, 4ug; Detection:17466-1-AP 1:300) with mouse brain tissue lysate 4000 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SKAR Polyclonal antibody (17466-1-AP)

    IF Staining of HeLa using 17466-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17466-1-AP (SKAR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, HepG2 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17466-1-AP targets SKAR in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SKAR fusion protein Ag11338 相同性解析による交差性が予測される生物種
Full Name polymerase (DNA-directed), delta interacting protein 3
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC019643
Gene symbol SKAR
Gene ID (NCBI) 84271
RRIDAB_2166620
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SKAR antibody 17466-1-APDownload protocol
IF protocol for SKAR antibody 17466-1-APDownload protocol
IP protocol for SKAR antibody 17466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

PDIP46 (DNA polymerase δ interacting protein 46) is an activating factor for human DNA polymerase δ.

Authors - Xiaoxiao Wang
View Article
humanWB,IF

Sci Rep

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Authors - S Prpar Mihevc
View Article
humanWB

Am J Cancer Res

Inhibition of mTORC1 signaling sensitizes hepatocellular carcinoma cells to glycolytic stress.

Authors - Xin Zhao
View Article