Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 17466-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 17466-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17466-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17466-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 17466-1-AP
IP result of anti-SKAR (IP:17466-1-AP, 4ug; Detection:17466-1-AP 1:300) with mouse brain tissue lysate 4000 ug.
IHC staining of human stomach using 17466-1-AP
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17466-1-AP (SKAR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 17466-1-AP
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17466-1-AP (SKAR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 17466-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17466-1-AP (SKAR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SKAR Polyclonal antibody

SKAR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17466-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

POLDIP3, Polymerase delta-interacting protein 3, PDIP46, PDIP3, p46

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, HepG2 cells, mouse brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 1 publications below

Product Information

17466-1-AP targets SKAR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SKAR fusion protein Ag11338 相同性解析による交差性が予測される生物種
Full Name	polymerase (DNA-directed), delta interacting protein 3
Calculated molecular weight	46 kDa
Observed molecular weight	46 kDa
GenBank accession number	BC019643
Gene Symbol	SKAR
Gene ID (NCBI)	84271
RRID	AB_2166620
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BY77
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SKAR antibody 17466-1-AP	Download protocol
IHC protocol for SKAR antibody 17466-1-AP	Download protocol
IF protocol for SKAR antibody 17466-1-AP	Download protocol
IP protocol for SKAR antibody 17466-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncotarget PDIP46 (DNA polymerase δ interacting protein 46) is an activating factor for human DNA polymerase δ. Authors - Xiaoxiao Wang View Article
human	WB,IF	Sci Rep TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins. Authors - S Prpar Mihevc View Article
human	WB	Am J Cancer Res Inhibition of mTORC1 signaling sensitizes hepatocellular carcinoma cells to glycolytic stress. Authors - Xin Zhao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 17466-1-AP

WB analysis of A549 using 17466-1-AP

WB analysis of HepG2 using 17466-1-AP

WB analysis of mouse brain using 17466-1-AP

IP experiment of mouse brain using 17466-1-AP

IHC staining of human stomach using 17466-1-AP