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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLG Recombinant antibody (83670-1-RR)

    WB analysis using 83670-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83670-1-RR (POLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using POLG Recombinant antibody (83670-1-RR)

    WB analysis of rat testis using 83670-1-RR

    rat testis tissue were subjected to SDS PAGE followed by western blot with 83670-1-RR (POLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using POLG Recombinant antibody (83670-1-RR)

    WB analysis of rat brain using 83670-1-RR

    rat brain tissue were subjected to SDS PAGE followed by western blot with 83670-1-RR (POLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83670-1-RR

    Affinity and Kinetic Characterization of 83670-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83670-1-RR against Human POLG were performed. The affinity constant is below 1 pM.

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POLG Recombinant antibody

POLG Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83670-1-RR
Clone No.240441D2

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

EC:3.1.11.-, EC:2.7.7.7, DNA polymerase subunit gamma-1, 5'-deoxyribose-phosphate lyase, 3'-5' exodeoxyribonuclease

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, rat brain tissue, rat testis tissue, HepG2 cells, HEK-293T cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83670-1-RR targets POLG in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen POLG fusion protein Ag5256 相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), gamma
Calculated molecular weight 140 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC042571
Gene Symbol POLG
Gene ID (NCBI) 5428
RRIDAB_3671275
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP54098
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POLG (DNA polymerase gamma) is composed of a C-terminal polymerase ('pol') domain and an amino-terminal exonuclease ('exo') domain. The exo domain increases the fidelity of mitochondrial DNA replication by conferring a proofreading activity to the enzyme (PMID: 12210792). Catalytic subunit of POLG solely responsible for replication of mitochondrial DNA (mtDNA). Replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers and the four deoxyribonucleoside triphosphates as substrates (PMID:11477093; 11897778; 15917273; 19837034; 9558343).

Protocols

Product Specific Protocols
WB protocol for POLG antibody 83670-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols