Validation Data Gallery
Tested Applications
Positive WB detected in | BxPC-3 cells, HeLa cells, COLO 320 cells, HEK-293T cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
28133-1-AP targets POLH in WB, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | POLH fusion protein Ag27973 相同性解析による交差性が予測される生物種 |
Full Name | polymerase (DNA directed), eta |
Calculated molecular weight | 713aa,78 kDa; 414aa,46 kDa |
Observed molecular weight | 78-85 kDa |
GenBank accession number | BC015742 |
Gene Symbol | POLH |
Gene ID (NCBI) | 5429 |
RRID | AB_2881070 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y253 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for POLH antibody 28133-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |