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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POLK Polyclonal antibody

POLK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14455-1-AP

Synonyms

DINB protein, DINB1, DINP, DNA polymerase kappa, POLK, POLQ

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLK Polyclonal antibody (14455-1-AP)

    WB analysis using 14455-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14455-1-AP (POLK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14455-1-AP targets POLK in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLK fusion protein Ag6034 相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed) kappa
Calculated molecular weight 99 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC050718
Gene symbol POLK
Gene ID (NCBI) 51426
RRIDAB_2165839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POLK, also named as DINB1, belongs to the DNA polymerase type-Y family. POLK is a DNA polymerase specifically involved in DNA repair. POLK Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, POLK inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK is ubiquitously expressed in tissues, but is most abundant in testis, adrenal gland and ovary (PMID: 15661663). POLK has 8 isoforms with the molecular mass of 42-99 kDa.

Protocols

Product Specific Protocols
WB protocol for POLK antibody 14455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols