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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POLM Polyclonal antibody

POLM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14873-1-AP

Synonyms

DNA polymerase mu, Pol Mu, POLM, polymerase (DNA directed), mu, Tdt N

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using POLM Polyclonal antibody (14873-1-AP)

    WB analysis of Jurkat using 14873-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14873-1-AP (POLM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using POLM Polyclonal antibody (14873-1-AP)

    WB analysis of Jurkat using 14873-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14873-1-AP (POLM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using POLM Polyclonal antibody (14873-1-AP)

    IHC staining of human spleen using 14873-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using POLM Polyclonal antibody (14873-1-AP)

    IHC staining of human spleen using 14873-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using POLM Polyclonal antibody (14873-1-AP)

    IHC staining of human lung using 14873-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using POLM Polyclonal antibody (14873-1-AP)

    IHC staining of human lung using 14873-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using POLM Polyclonal antibody (14873-1-AP)

    IHC staining of human ovary using 14873-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using POLM Polyclonal antibody (14873-1-AP)

    IHC staining of human ovary using 14873-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using POLM Polyclonal antibody (14873-1-AP)

    IHC staining of human kidney using 14873-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using POLM Polyclonal antibody (14873-1-AP)

    IHC staining of human kidney using 14873-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using POLM Polyclonal antibody (14873-1-AP)

    IHC staining of human testis using 14873-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using POLM Polyclonal antibody (14873-1-AP)

    IHC staining of human testis using 14873-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using POLM Polyclonal antibody (14873-1-AP)

    IHC staining of human skin using 14873-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using POLM Polyclonal antibody (14873-1-AP)

    IHC staining of human skin using 14873-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using POLM Polyclonal antibody (14873-1-AP)

    IHC staining of human brain using 14873-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using POLM Polyclonal antibody (14873-1-AP)

    IHC staining of human brain using 14873-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman spleen tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14873-1-AP targets POLM in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLM fusion protein Ag6642 相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), mu
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC062590
Gene symbol POLM
Gene ID (NCBI) 27434
RRIDAB_2237321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for POLM antibody 14873-1-APDownload protocol
IHC protocol for POLM antibody 14873-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols