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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POLQ Polyclonal antibody

POLQ Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28590-1-AP

Synonyms

DKFZp781A0112, DNA polymerase theta variant, Homo sapiens (Human), POLH, POLQ, PRO0327

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLQ Polyclonal antibody (28590-1-AP)

    WB analysis using 28590-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using POLQ Polyclonal antibody (28590-1-AP)

    WB analysis using 28590-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using POLQ Polyclonal antibody (28590-1-AP)

    IF Staining of A375 using 28590-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using POLQ Polyclonal antibody (28590-1-AP)

    IF Staining of A431 using 28590-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHuH-7 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inA375 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28590-1-AP targets POLQ in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLQ fusion protein Ag29643 相同性解析による交差性が予測される生物種
Full Name polymerase (DNA directed), theta
Calculated molecular weight 290 kDa
Observed molecular weight250~290 kDa, 70~110 kDa
GenBank accession numberNM_199420
Gene symbol POLQ
Gene ID (NCBI) 10721
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA Polymerase Theta (POLQ) is a DNA polymerase involved in error-prone translesion DNA synthesis (TLS) and error-prone repair of DNA double-strand breaks (DSBs). The enzyme has several unique properties, including low fidelity and the ability to insert and extend past abasic sites and thymine glycol lesions. Accumulating evidence showed that POLQ participates in the development and progression of human cancers because of its critical functions in the repair of genomic double-strand breaks (PMID: 34517891, 27264557). The POLQ cDNA encodes a polypeptide of 2592 amino acids with a predicted mass of 290 kDa, but POLQ is readily proteolyzed with smaller bands at approximately 70 kDa and 110 kDa (PMID: 17334634, 14576298).

Protocols

Product Specific Protocols
WB protocol for POLQ antibody 28590-1-APDownload protocol
IF protocol for POLQ antibody 28590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols