Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, mouse lung tissue |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
12254-1-AP targets POLR1D in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | POLR1D fusion protein Ag2904 相同性解析による交差性が予測される生物種 |
| Full Name | polymerase (RNA) I polypeptide D, 16kDa |
| Calculated molecular weight | 16 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC015319 |
| Gene Symbol | POLR1D |
| Gene ID (NCBI) | 51082 |
| RRID | AB_10640535 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P0DPB6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POLR1D antibody 12254-1-AP | Download protocol |
| IP protocol for POLR1D antibody 12254-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|