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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using POLR1D Polyclonal antibody (16678-1-AP)

    WB analysis of Jurkat using 16678-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16678-1-AP (POLR1D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using POLR1D Polyclonal antibody (16678-1-AP)

    WB analysis of HeLa using 16678-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16678-1-AP (POLR1D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using POLR1D Polyclonal antibody (16678-1-AP)

    WB analysis of HepG2 using 16678-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16678-1-AP (POLR1D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using POLR1D Polyclonal antibody (16678-1-AP)

    IHC staining of human ovary cancer using 16678-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16678-1-AP (POLR1D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POLR1D Polyclonal antibody (16678-1-AP)

    IF Staining of HepG2 using 16678-1-AP

    Immunofluorescent analysis of HepG2 cells using 16678-1-AP (POLR1D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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POLR1D Polyclonal antibody

POLR1D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16678-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RPA16, POLR1C, hRPA19, DNA-directed RNA polymerases I and III subunit RPAC2, DNA-directed RNA polymerase I subunit D

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16678-1-AP targets POLR1D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR1D fusion protein Ag10000 相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) I polypeptide D, 16kDa
Calculated molecular weight 15 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC000889
Gene Symbol POLR1D
Gene ID (NCBI) 51082
RRIDAB_2878298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0DPB6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for POLR1D antibody 16678-1-APDownload protocol
IHC protocol for POLR1D antibody 16678-1-APDownload protocol
IF protocol for POLR1D antibody 16678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols