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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POLR2A Recombinant antibody

POLR2A Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240869E9

Cat no : 84357-1-RR

Synonyms

EC:2.7.7.48, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A, 3'-5' exoribonuclease

Validation Data Gallery

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  • Western Blot (WB) analysis of COS-7 cells using POLR2A Recombinant antibody (84357-1-RR)

    WB analysis of COS-7 using 84357-1-RR

    COS-7 cells were subjected to SDS PAGE followed by western blot with 84357-1-RR (POLR2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using POLR2A Recombinant antibody (84357-1-RR)

    IHC staining of mouse pancreas using 84357-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84357-1-RR (POLR2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POLR2A Recombinant antibody (84357-1-RR)

    IF Staining of HepG2 using 84357-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR2A antibody (84357-1-RR, Clone: 240869E9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inCOS-7 cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84357-1-RR targets POLR2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, monkey samples.

Tested Reactivity human, monkey
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Calculated molecular weight 217 kDa
Observed molecular weight245 kDa
GenBank accession numberNM_000937
Gene symbol POLR2A
Gene ID (NCBI) 5430
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA-directed RNA polymerase II subunit RPB1(POLR2A), is part of the core element of the basic RNA polymerase II transcription mechanism. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for the assembly of factors that regulate transcription initiation, elongation, termination, and mRNA processing.

Protocols

Product Specific Protocols
WB protocol for POLR2A antibody 84357-1-RRDownload protocol
IHC protocol for POLR2A antibody 84357-1-RRDownload protocol
IF protocol for POLR2A antibody 84357-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols