Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| ChIP | See 1 publications below |
Product Information
20370-1-AP targets POLR2B-Specific in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | polymerase (RNA) II (DNA directed) polypeptide B, 140kDa |
| Calculated molecular weight | 134 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | NM_000938 |
| Gene Symbol | POLR2B |
| Gene ID (NCBI) | 5431 |
| RRID | AB_10693617 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30876 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
POLR2B belongs to the RNA polymerase beta chain family. It is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is the second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. POLR2B proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. The antibody is specific to POLR2B.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POLR2B-Specific antibody 20370-1-AP | Download protocol |
| IHC protocol for POLR2B-Specific antibody 20370-1-AP | Download protocol |
| IP protocol for POLR2B-Specific antibody 20370-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol MCP-1 facilitates VEGF production by removing miR-374b-5p blocking of VEGF mRNA translation | ||
Cell Death Dis M6A-methylated circPOLR2B forms an R-loop and regulates the biological behavior of glioma stem cells through positive feedback loops |