POLR2C Polyclonal antibody

Name: POLR2C Polyclonal antibody
Brand: Proteintech
SKU: 13428-1-AP

POLR2C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13428-1-AP

Synonyms

hRPB33, hsRPB3, POLR2C, RNA polymerase II subunit 3, RNA polymerase II subunit B3, RPB3, RPB31, RPB33

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of mouse kidney using 13428-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13428-1-AP (POLR2C Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13428-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13428-1-AP (POLR2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 13428-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13428-1-AP (POLR2C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 13428-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13428-1-AP (POLR2C Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13428-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13428-1-AP (POLR2C Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 13428-1-AP
IP result of anti-POLR2C (IP:13428-1-AP, 4ug; Detection:13428-1-AP 1:500) with HeLa cells lysate 2000ug.
IHC staining of human cervical cancer using 13428-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13428-1-AP (POLR2C antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human cervical cancer using 13428-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13428-1-AP (POLR2C antibody) at dilution of 1:100 (under 40x lens).

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Cat No. 13428-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse kidney tissue, HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

13428-1-AP targets POLR2C in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	POLR2C fusion protein Ag4228 相同性解析による交差性が予測される生物種
Full Name	polymerase (RNA) II (DNA directed) polypeptide C, 33kDa
Calculated molecular weight	275 aa, 33 kDa
Observed molecular weight	33 kDa
GenBank accession number	BC028157
Gene symbol	POLR2C
Gene ID (NCBI)	5432
RRID	AB_2167653
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for POLR2C antibody 13428-1-AP	Download protocol
IHC protocol for POLR2C antibody 13428-1-AP	Download protocol
IP protocol for POLR2C antibody 13428-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits Authors - Yuanjun Li View Article
mouse	WB	Mol Cell Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity Authors - Yuanjun Li View Article
human	WB	Biomed Microdevices Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria. Authors - Hao-Peng Lin View Article
mouse		Genome Biol Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture Authors - Yongpeng Jiang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POLR2C Polyclonal antibody

POLR2C Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of mouse kidney using 13428-1-AP

WB analysis of HeLa using 13428-1-AP

WB analysis of mouse kidney using 13428-1-AP

WB analysis of mouse kidney using 13428-1-AP

WB analysis of HeLa using 13428-1-AP

IP experiment of HeLa using 13428-1-AP

IHC staining of human cervical cancer using 13428-1-AP

IHC staining of human cervical cancer using 13428-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits

Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity

Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria.

Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture