RPB5 Polyclonal antibody

Name: RPB5 Polyclonal antibody
Brand: Proteintech
SKU: 15217-1-AP

RPB5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15217-1-AP

Synonyms

hRPB25, hsRPB5, POLR2E, RPABC1, RPB5, RPB5 homolog, XAP4

Validation Data Gallery

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AllWBIPIHC

WB analysis using 15217-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15217-1-AP (RPB5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15217-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15217-1-AP (POLR2E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15217-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15217-1-AP (POLR2E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15217-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15217-1-AP (POLR2E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 15217-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 15217-1-AP (POLR2E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15217-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15217-1-AP (POLR2E antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 15217-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 15217-1-AP (RPB5 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 15217-1-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 15217-1-AP (RPB5 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 15217-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15217-1-AP (RPB5 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15217-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15217-1-AP (RPB5 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15217-1-AP
IP result of anti-RPB5 (IP:15217-1-AP, 4ug; Detection:15217-1-AP 1:500) with HeLa cells lysate 880ug.
IHC staining of human breast cancer using 15217-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15217-1-AP (RPB5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15217-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15217-1-AP (RPB5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 15217-1-AP

Size/Concentration

ご購入について

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, HeLa cells, MCF-7 cells, HepG2 cells, rat liver tissue, rat spleen tissue, mouse spleen tissue, mouse liver tissue, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

15217-1-AP targets RPB5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RPB5 fusion protein Ag7386 相同性解析による交差性が予測される生物種
Full Name	polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
Calculated molecular weight	25 kDa
Observed molecular weight	23-25 kDa
GenBank accession number	BC004441
Gene symbol	RPB5
Gene ID (NCBI)	5434
RRID	AB_2299932
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. polymerase (RNA) II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E (Polymerase (RNA) II (DNA directed) polypeptide E) /RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process. The observed molecular weight of POLR2E is about 25 kDa.

Protocols

Product Specific Protocols
WB protocol for RPB5 antibody 15217-1-AP	Download protocol
IHC protocol for RPB5 antibody 15217-1-AP	Download protocol
IP protocol for RPB5 antibody 15217-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits Authors - Yuanjun Li View Article
human	WB	Mol Cell SPT5 stabilization of promoter-proximal RNA polymerase II. Authors - Yuki Aoi View Article
mouse	WB	Mol Cell Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity Authors - Yuanjun Li View Article
human	WB	Oncogene RMP promotes venous metastases of hepatocellular carcinoma through promoting IL-6 transcription. Authors - J Zhang View Article
mouse		Genome Biol Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture Authors - Yongpeng Jiang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPB5 Polyclonal antibody

RPB5 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 15217-1-AP

WB analysis of HeLa using 15217-1-AP

WB analysis of HeLa using 15217-1-AP

WB analysis of MCF-7 using 15217-1-AP

WB analysis of A549 using 15217-1-AP

WB analysis of HepG2 using 15217-1-AP

WB analysis of rat liver using 15217-1-AP

WB analysis of rat spleen using 15217-1-AP

WB analysis of mouse spleen using 15217-1-AP

WB analysis of mouse liver using 15217-1-AP

IP experiment of HeLa using 15217-1-AP

IHC staining of human breast cancer using 15217-1-AP

IHC staining of human breast cancer using 15217-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits

SPT5 stabilization of promoter-proximal RNA polymerase II.

Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity

RMP promotes venous metastases of hepatocellular carcinoma through promoting IL-6 transcription.

Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture