Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HL-60 using 15086-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15086-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 15086-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 15086-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15086-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15086-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15086-1-AP (POLR2H antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 15086-1-AP
IP result of anti-POLR2H (IP:15086-1-AP, 4ug; Detection:15086-1-AP 1:500) with mouse liver tissue lysate 6000 ug.
IHC staining of human breast cancer using 15086-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 15086-1-AP (POLR2H antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human breast cancer using 15086-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 15086-1-AP (POLR2H antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 15086-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15086-1-AP (POLR2H antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

POLR2H Polyclonal antibody

POLR2H Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15086-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

RPABC3, hsRPB8, hRPB8, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HL-60 cells, mouse kidney tissue, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

15086-1-AP targets POLR2H in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	POLR2H fusion protein Ag7166 相同性解析による交差性が予測される生物種
Full Name	polymerase (RNA) II (DNA directed) polypeptide H
Calculated molecular weight	17 kDa
Observed molecular weight	17 kDa
GenBank accession number	BC000739
Gene Symbol	POLR2H
Gene ID (NCBI)	5437
RRID	AB_2167826
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P52434
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

POLR2H, also known as hRPB8, is a 150 amino acid protein, which localizes in nucleolus and is expressed in 228 organ(s), highest expression level in mucosa of transverse colon. POLR2H. POLR2H is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR2H is a common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively

Protocols

Product Specific Protocols
WB protocol for POLR2H antibody 15086-1-AP	Download protocol
IHC protocol for POLR2H antibody 15086-1-AP	Download protocol
IF protocol for POLR2H antibody 15086-1-AP	Download protocol
IP protocol for POLR2H antibody 15086-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Cell Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity Authors - Yuanjun Li View Article
human	IHC	Oncol Lett Identification of the key genes and pathways in prostate cancer. Authors - Shutong Fan View Article
mouse		Genome Biol Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture Authors - Yongpeng Jiang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HL-60 using 15086-1-AP

WB analysis of MCF-7 using 15086-1-AP

WB analysis of rat liver using 15086-1-AP

WB analysis of mouse kidney using 15086-1-AP

WB analysis of mouse liver using 15086-1-AP

WB analysis of HeLa using 15086-1-AP

IP experiment of mouse liver using 15086-1-AP

IHC staining of human breast cancer using 15086-1-AP