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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLR2H Recombinant antibody (83439-1-RR)

    WB analysis using 83439-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83439-1-RR (POLR2H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83439-1-RR

    Affinity and Kinetic Characterization of 83439-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83439-1-RR against Human POLR2H were performed. The affinity constant is below 1 pM.

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POLR2H Recombinant antibody

POLR2H Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83439-1-RR
Clone No.240410F9

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

hsRPB8, hRPB8, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide, DNA-directed RNA polymerase II subunit H

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HL-60 cells, HepG2 cells, NIH/3T3 cells, Jurkat cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83439-1-RR targets POLR2H in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen POLR2H fusion protein Ag7166 相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) II (DNA directed) polypeptide H
Calculated molecular weight 17 kDa
Observed molecular weight15-17 kDa
GenBank accession numberBC000739
Gene Symbol POLR2H
Gene ID (NCBI) 5437
RRIDAB_3671080
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52434
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POLR2H, also known as hRPB8, is a 150 amino acid protein, which localizes in nucleolus and is expressed in 228 organ(s), highest expression level in mucosa of transverse colon. POLR2H. POLR2H is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR2H is a common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively

Protocols

Product Specific Protocols
WB protocol for POLR2H antibody 83439-1-RRDownload protocol
FC protocol for POLR2H antibody 83439-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols