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POLR2I Polyclonal antibody
POLR2I Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 17270-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells |
| Positive IHC detected in | mouse stomach tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
17270-1-AP targets POLR2I in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | POLR2I fusion protein Ag11027 相同性解析による交差性が予測される生物種 |
| Full Name | polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa |
| Calculated molecular weight | 125 aa, 15 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | BC017112 |
| Gene symbol | POLR2I |
| Gene ID (NCBI) | 5438 |
| RRID | AB_10836936 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
POLR2I, also known as RPB9 or hRPB14.5, is a small core subunit of RNA polymerase II involved in DNA repair and many transcription-related processes (PMID: 21450810). It is expressed in many tissues and may play a key role in the pathological process of hypertensive nephropathy (PMID: 34422001).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POLR2I antibody 17270-1-AP | Download protocol |
| IHC protocol for POLR2I antibody 17270-1-AP | Download protocol |
| IF protocol for POLR2I antibody 17270-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity | ||
EMBO Rep The RNA polymerase II subunit Rpb9 activates ATG1 transcription and autophagy
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