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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLR3C Recombinant antibody (84367-6-RR)

    WB analysis using 84367-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84367-6-RR (POLR3C antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84367-6-RR

    Affinity and Kinetic Characterization of 84367-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84367-6-RR against Human POLR3C were performed. The affinity constant is below 1 pM.

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POLR3C Recombinant antibody

POLR3C Recombinant Antibody for WB, ELISA
Cat No. 84367-6-RR
Clone No.241402A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

RPC62, RPC3, RNA polymerase III subunit C3, DNA-directed RNA polymerase III subunit RPC3, DNA-directed RNA polymerase III subunit C

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84367-6-RR targets POLR3C in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen POLR3C fusion protein Ag27311 相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) III (DNA directed) polypeptide C (62kD)
Calculated molecular weight 61 kDa
Observed molecular weight58 kDa
GenBank accession numberBC002586
Gene Symbol POLR3C
Gene ID (NCBI) 10623
RRIDAB_3671903
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BUI4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mutations in the POLR3A, POLR3C, POLR3E, and POLR3F subunits are associated with susceptibility to varicella zoster virus-induced encephalitis and pneumonitis (PMID: 34395528).

Protocols

Product Specific Protocols
WB protocol for POLR3C antibody 84367-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols