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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POLR3G Polyclonal antibody

POLR3G Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 24701-1-AP

Synonyms

POLR3G, RNA polymerase III subunit C7, RPC32, RPC7

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using POLR3G Polyclonal antibody (24701-1-AP)

    WB analysis of HeLa using 24701-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24701-1-AP (POLR3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using POLR3G Polyclonal antibody (24701-1-AP)

    WB analysis of K-562 using 24701-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24701-1-AP (POLR3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using POLR3G Polyclonal antibody (24701-1-AP)

    WB analysis of HEK-293 using 24701-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24701-1-AP (POLR3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using POLR3G Polyclonal antibody (24701-1-AP)

    IP experiment of HeLa using 24701-1-AP

    IP result of anti-POLR3G (IP:24701-1-AP, 3ug; Detection:24701-1-AP 1:500) with HeLa cells lysate 2800ug.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 24701-1-AP is POLR3G Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR3G fusion protein Ag19872 相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) III (DNA directed) polypeptide G (32kD)
Calculated molecular weight 223 aa, 26 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC141649
Gene symbol POLR3G
Gene ID (NCBI) 10622
RRIDAB_2879681
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POLR3G also named as RNA polymerase III subunit C7 is a 223 amino acid protein, which belongs to eukaryotic RPC7 RNA polymerase subunit family. The molecular weight of POLR3G is 26 kDa. POLR3G localizes in the nucleus and is a component of the RNA polymerase III complex, which may be involved either in the recruitment and stablization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation.

Protocols

Product Specific Protocols
WB protocol for POLR3G antibody 24701-1-APDownload protocol
IP protocol for POLR3G antibody 24701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hum Genet

Biallelic variants in GTF3C5, a regulator of RNA polymerase III-mediated transcription, cause a multisystem developmental disorder

Authors - Aiko Iwata-Otsubo
View Article