Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
16423-1-AP targets POLR3H in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | POLR3H fusion protein Ag9624 相同性解析による交差性が予測される生物種 |
| Full Name | polymerase (RNA) III (DNA directed) polypeptide H (22.9kD) |
| Calculated molecular weight | 175 aa, 20 kDa |
| Observed molecular weight | 25-30 kDa |
| GenBank accession number | BC017248 |
| Gene Symbol | POLR3H |
| Gene ID (NCBI) | 171568 |
| RRID | AB_2878257 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y535 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POLR3H antibody 16423-1-AP | Download protocol |
| IF protocol for POLR3H antibody 16423-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|