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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POLR3H Polyclonal antibody

POLR3H Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16423-1-AP

Synonyms

RPC8, KIAA1665, DNA-directed RNA polymerase III subunit RPC8, DNA-directed RNA polymerase III subunit H

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using POLR3H Polyclonal antibody (16423-1-AP)

    WB analysis of HeLa using 16423-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16423-1-AP (POLR3H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using POLR3H Polyclonal antibody (16423-1-AP)

    WB analysis of HeLa using 16423-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16423-1-AP (POLR3H Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POLR3H Polyclonal antibody (16423-1-AP)

    IF Staining of HeLa using 16423-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16423-1-AP (POLR3H antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16423-1-AP targets POLR3H in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR3H fusion protein Ag9624 相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) III (DNA directed) polypeptide H (22.9kD)
Calculated molecular weight 175 aa, 20 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC017248
Gene symbol POLR3H
Gene ID (NCBI) 171568
RRIDAB_2878257
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for POLR3H antibody 16423-1-APDownload protocol
IF protocol for POLR3H antibody 16423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

Subcellular localization shapes the fate of RNA polymerase III

Authors - Kai Tian
View Article