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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POLR3K Polyclonal antibody

POLR3K Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11084-1-AP

Synonyms

HsC11p, hRPC11, DNA-directed RNA polymerase III subunit RPC10, DNA-directed RNA polymerase III subunit K, C11 RNP3

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using POLR3K Polyclonal antibody (11084-1-AP)

    IHC staining of mouse testis using 11084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11084-1-AP (POLR3K antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using POLR3K Polyclonal antibody (11084-1-AP)

    IHC staining of mouse testis using 11084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11084-1-AP (POLR3K antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using POLR3K Polyclonal antibody (11084-1-AP)

    IHC staining of human gliomas using 11084-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11084-1-AP (POLR3K antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using POLR3K Polyclonal antibody (11084-1-AP)

    IHC staining of mouse testis using 11084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11084-1-AP (POLR3K antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using POLR3K Polyclonal antibody (11084-1-AP)

    IHC staining of mouse testis using 11084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11084-1-AP (POLR3K antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POLR3K Polyclonal antibody (11084-1-AP)

    IF Staining of HepG2 using 11084-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR3K antibody (11084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POLR3K Polyclonal antibody (11084-1-AP)

    IF Staining of HepG2 using 11084-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11084-1-AP (POLR3K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inmouse testis tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11084-1-AP targets POLR3K in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR3K fusion protein Ag1564 相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
Calculated molecular weight 12 kDa
GenBank accession numberBC011932
Gene symbol POLR3K
Gene ID (NCBI) 51728
RRIDAB_2165725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for POLR3K antibody 11084-1-APDownload protocol
IF protocol for POLR3K antibody 11084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols