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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POLRMT Polyclonal antibody

POLRMT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17748-1-AP

Synonyms

MTRPOL, MTRNAP, h mtRPOL, EC:2.7.7.6, DNA-directed RNA polymerase, mitochondrial

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLRMT Polyclonal antibody (17748-1-AP)

    WB analysis using 17748-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17748-1-AP (POLRMT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using POLRMT Polyclonal antibody (17748-1-AP)

    WB analysis of COLO 320 using 17748-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17748-1-AP (POLRMT Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using POLRMT Polyclonal antibody (17748-1-AP)

    IHC staining of human ovary cancer using 17748-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17748-1-AP (POLRMT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using POLRMT Polyclonal antibody (17748-1-AP)

    IF Staining of U2OS using 17748-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using POLRMT antibody (17748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHepG2 cells, COLO 320 cells, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17748-1-AP targets POLRMT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLRMT fusion protein Ag12012 相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) mitochondrial (DNA directed)
Calculated molecular weight 1230 aa, 139 kDa
Observed molecular weight 130-139 kDa
GenBank accession numberBC098387
Gene symbol POLRMT
Gene ID (NCBI) 5442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitochondrial DNA-dependent RNA polymerase (POLRMT) is responsible for the transcription of mitochondrial genome encoding key components of oxidative phosphorylation. Structurally, POLRMT comprises four main domains: the N-terminal extension, a pentatricopeptide repeat (PPR) domain, the N-terminal domain, and the C-terminal domain(PMID: 33602924). POLRMT requires transcription factor B2M (TFB2M) and transcription factor AM (TFAM) for mitochondrial RNA transcription. POLRMT is overexpressed at both the mRNA and protein level in several cancers, including breast, skin, lung, endometrial cancer, and osteosarcomas(PMID: 37371693).

Protocols

Product Specific Protocols
WB protocol for POLRMT antibody 17748-1-APDownload protocol
IHC protocol for POLRMT antibody 17748-1-APDownload protocol
IF protocol for POLRMT antibody 17748-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols