Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HCT 116 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 6 publications below |
Product Information
15645-1-AP targets POM121 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | POM121 fusion protein Ag8177 相同性解析による交差性が予測される生物種 |
| Full Name | POM121 membrane glycoprotein (rat) |
| Calculated molecular weight | 984 aa, 99 kDa |
| Observed molecular weight | 140-150 kDa |
| GenBank accession number | BC008794 |
| Gene Symbol | POM121 |
| Gene ID (NCBI) | 9883 |
| RRID | AB_2878160 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96HA1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
POM121 is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Catalog#15645-1-AP do well in identifying the nuclear envelope in immunofluorescence experiments.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POM121 antibody 15645-1-AP | Download protocol |
| IHC protocol for POM121 antibody 15645-1-AP | Download protocol |
| IF protocol for POM121 antibody 15645-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Neurosci Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway. | ||
Elife Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2. | ||
Cancer Biomark POM121 promotes proliferation and metastasis in non-small-cell lung cancer through TGF-β/SMAD and PI3K/AKT pathways
| ||
bioRxiv Altered nuclear envelope homeostasis is a key pathogenic event in C9ORF72-linked ALS/FTD |