POMC Monoclonal antibody

POMC Monoclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse and More (2)

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3C12C7

Cat no : 66358-1-Ig

Synonyms

Beta-endorphin, Beta LPH Lipotropin gamma, Adrenocorticotropic hormone, ACTH Melanotropin alpha, ACTH


☆対象製品が30%オフ!組換えタンパク質&免染用試薬 >>> キャンペーン実施中!


Tested Applications

Positive IHC detected inhuman pituitary tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66358-1-Ig targets POMC in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen POMC fusion protein Ag17311 相同性解析による交差性が予測される生物種
Full Name proopiomelanocortin
Calculated molecular weight 267 aa, 29 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC065832
Gene symbol POMC
Gene ID (NCBI) 5443
RRIDAB_2881737
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.

Protocols

Product Specific Protocols
IHC protocol for POMC antibody 66358-1-IgDownload protocol
IF protocol for POMC antibody 66358-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Elife

Single-cell transcriptome analysis identifies a unique tumor cell type producing multiple hormones in ectopic ACTH and CRH secreting pheochromocytoma.

Authors - Xuebin Zhang
mouseIF

Biomed Pharmacother

919 syrup inhibits ROS-mediated leptin-induced anorexia by activating PPARγ and improves gut flora abnormalities.

Authors - Jing-Wei Xing
ratIF

Sci Rep

Gold nanoclusters eliminate obesity induced by antipsychotics.

Authors - Meng He
mouseIF

Biochem Biophys Res Commun

Elongator regulates the melanocortin satiety pathway.

Authors - Joseph Walters
ratIF

Reprod Sci

FoxO1 Regulates Neuropeptide Y and Pro-opiomelanocortin in the Hypothalamus of Rat Offspring Small for Gestational Age.

Authors - Lifang Zhang
pigIHC

Food Addit Contam Part A Chem Anal

FumDSB can alleviate the inflammatory response induced by fumonisin B1 in growing pigs.

Authors - Quancheng Liu