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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PON3 Polyclonal antibody (31793-1-AP)

    WB analysis using 31793-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31793-1-AP (PON3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PON3 Polyclonal antibody (31793-1-AP)

    IHC staining of human liver using 31793-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using PON3 Polyclonal antibody (31793-1-AP)

    IHC staining of human liver using 31793-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using PON3 Polyclonal antibody (31793-1-AP)

    IHC staining of rat liver using 31793-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using PON3 Polyclonal antibody (31793-1-AP)

    IHC staining of rat liver using 31793-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PON3 Polyclonal antibody (31793-1-AP)

    IHC staining of mouse liver using 31793-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PON3 Polyclonal antibody (31793-1-AP)

    IHC staining of mouse liver using 31793-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31793-1-AP (PON3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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PON3 Polyclonal antibody

PON3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31793-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Paraoxonase 3 , Paraoxanase-3, EC:3.1.8.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue, mouse liver tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31793-1-AP targets PON3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PON3 fusion protein Ag36602 相同性解析による交差性が予測される生物種
Full Name paraoxonase 3
Calculated molecular weightNM_000940.2 40kDa，354aa
Observed molecular weight40 kDa
GenBank accession numberNM_000940.2
Gene Symbol PON3
Gene ID (NCBI) 5446
RRIDAB_3670115
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ15166
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PON3, a member of the paraoxonase family, is expressed primarily in the liver and encodes a protein that is secreted into the bloodstream and binds to high-density lipoprotein (HDL). It also rapidly hydrolyzes lactones and inhibits the oxidation of low-density lipoprotein (LDL), thereby slowing the onset and progression of atherosclerosis. In addition, PON3 is closely associated with hepatocellular carcinoma (HCC), and is a potential therapeutic target for HCC by inducing cell cycle arrest and further inhibiting HCC cell proliferation.

Protocols

Product Specific Protocols
WB protocol for PON3 antibody 31793-1-APDownload protocol
IHC protocol for PON3 antibody 31793-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols