POP1 Polyclonal antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 12029-1-AP (POP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

WB result of POP1 antibody (12029-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POP1 transfected HeLa cells. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

IP result of anti-POP1 (IP:12029-1-AP, 4ug; Detection:12029-1-AP 1:8000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12029-1-AP (POP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POP1 antibody (12029-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.