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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

POP4 Polyclonal antibody

POP4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15273-1-AP

Synonyms

RPP29, POP 4, hPOP4

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using POP4 Polyclonal antibody (15273-1-AP)

    WB analysis of HepG2 using 15273-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15273-1-AP (POP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using POP4 Polyclonal antibody (15273-1-AP)

    WB analysis of HeLa using 15273-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15273-1-AP (POP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POP4 Polyclonal antibody (15273-1-AP)

    IF Staining of HepG2 using 15273-1-AP

    Immunofluorescent analysis of HepG2 cells using 15273-1-AP (POP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15273-1-AP targets POP4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POP4 fusion protein Ag7387 相同性解析による交差性が予測される生物種
Full Name processing of precursor 4, ribonuclease P/MRP subunit (S. cerevisiae)
Calculated molecular weight 25 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC004438
Gene symbol POP4
Gene ID (NCBI) 10775
RRIDAB_2284033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for POP4 antibody 15273-1-APDownload protocol
IF protocol for POP4 antibody 15273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols