Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | COLO 320 cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse heart tissue, human skeletal muscle tissue, human placenta tissue, human testis tissue, human spleen tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 3 publications below |
Product Information
11800-1-AP targets POPDC3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | POPDC3 fusion protein Ag2375 相同性解析による交差性が予測される生物種 |
| Full Name | popeye domain containing 3 |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC022323 |
| Gene Symbol | POPDC3 |
| Gene ID (NCBI) | 64208 |
| RRID | AB_2166494 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBV1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The Popeye domain-containing (POPDC) gene family includes BVES/POPDC1, POPDC2 and POPDC3. POPDC proteins are highly evolutionarily conserved transmembrane proteins expressed in embryonic epithelium, heart and muscle. POPDC3 is a 291-amino acid protein containing three putative transmembrane domains. It has been reported that POPDC3 expression is reduced in gastric cancer, suggesting it might play an important role in the progression and metastases of gastric cancer. (PMID: 10882522; 20627872; 22654436)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POPDC3 antibody 11800-1-AP | Download protocol |
| IHC protocol for POPDC3 antibody 11800-1-AP | Download protocol |
| IP protocol for POPDC3 antibody 11800-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Carcinogenesis Frequent silencing of popeye domain-containing genes, BVES and POPDC3, is associated with promoter hypermethylation in gastric cancer.
| ||
Front Oncol Study on differentially expressed genes between stage M and stage MS neuroblastoma | ||
World J Gastroenterol Reduced Popdc3 expression correlates with high risk and poor survival in patients with gastric cancer. |