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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

POPDC3 Polyclonal antibody

POPDC3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11800-1-AP

Synonyms

POP3, POPDC3, popeye domain containing 3, Popeye protein 3, RP11 99L11.2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POPDC3 Polyclonal antibody (11800-1-AP)

    WB analysis using 11800-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11800-1-AP (POPDC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using POPDC3 Polyclonal antibody (11800-1-AP)

    WB analysis of COLO 320 using 11800-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 11800-1-AP (POPDC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using POPDC3 Polyclonal antibody (11800-1-AP)

    WB analysis of HEK-293 using 11800-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11800-1-AP (POPDC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using POPDC3 Polyclonal antibody (11800-1-AP)

    IP experiment of HeLa using 11800-1-AP

    IP result of anti-POPDC3 (IP:11800-1-AP, 3ug; Detection:11800-1-AP 1:1000) with HeLa cells lysate 220ug.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using POPDC3 Polyclonal antibody (11800-1-AP)

    IHC staining of mouse heart using 11800-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using POPDC3 Polyclonal antibody (11800-1-AP)

    IHC staining of mouse heart using 11800-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using POPDC3 Polyclonal antibody (11800-1-AP)

    IHC staining of mouse heart using 11800-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11800-1-AP (POPDC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using POPDC3 Polyclonal antibody (11800-1-AP)

    IHC staining of mouse heart using 11800-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11800-1-AP (POPDC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using POPDC3 Polyclonal antibody (11800-1-AP)

    IHC staining of human skeletal muscle using 11800-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11800-1-AP (POPDC3 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using POPDC3 Polyclonal antibody (11800-1-AP)

    IHC staining of human skeletal muscle using 11800-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11800-1-AP (POPDC3 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human placenta tissue using POPDC3 Polyclonal antibody (11800-1-AP)

    IHC staining of human placenta using 11800-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using POPDC3 Polyclonal antibody (11800-1-AP)

    IHC staining of human placenta using 11800-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using POPDC3 Polyclonal antibody (11800-1-AP)

    IHC staining of human testis using 11800-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using POPDC3 Polyclonal antibody (11800-1-AP)

    IHC staining of human testis using 11800-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using POPDC3 Polyclonal antibody (11800-1-AP)

    IHC staining of human spleen using 11800-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using POPDC3 Polyclonal antibody (11800-1-AP)

    IHC staining of human spleen using 11800-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using POPDC3 Polyclonal antibody (11800-1-AP)

    IHC staining of human lung using 11800-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using POPDC3 Polyclonal antibody (11800-1-AP)

    IHC staining of human lung using 11800-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 11800-1-AP (POPDC3 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse heart tissue, human skeletal muscle tissue, human placenta tissue, human testis tissue, human spleen tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11800-1-AP targets POPDC3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POPDC3 fusion protein Ag2375 相同性解析による交差性が予測される生物種
Full Name popeye domain containing 3
Calculated molecular weight 34 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC022323
Gene symbol POPDC3
Gene ID (NCBI) 64208
RRIDAB_2166494
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Popeye domain-containing (POPDC) gene family includes BVES/POPDC1, POPDC2 and POPDC3. POPDC proteins are highly evolutionarily conserved transmembrane proteins expressed in embryonic epithelium, heart and muscle. POPDC3 is a 291-amino acid protein containing three putative transmembrane domains. It has been reported that POPDC3 expression is reduced in gastric cancer, suggesting it might play an important role in the progression and metastases of gastric cancer. (PMID: 10882522; 20627872; 22654436)

Protocols

Product Specific Protocols
WB protocol for POPDC3 antibody 11800-1-APDownload protocol
IHC protocol for POPDC3 antibody 11800-1-APDownload protocol
IP protocol for POPDC3 antibody 11800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Carcinogenesis

Frequent silencing of popeye domain-containing genes, BVES and POPDC3, is associated with promoter hypermethylation in gastric cancer.

Authors - Kim Mirang M
  • KD Validated
View Article
humanIHC

Front Oncol

Study on differentially expressed genes between stage M and stage MS neuroblastoma

Authors - Yuying Wu
View Article
humanIHC

World J Gastroenterol

Reduced Popdc3 expression correlates with high risk and poor survival in patients with gastric cancer.

Authors - Luo Deng D
View Article