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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse ovary tissue using POTEA-Specific Recombinant antibody (84298-1-RR)

    IHC staining of mouse ovary using 84298-1-RR (same clone as 84298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 84298-1-RR (POTEA-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84298-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POTEA-Specific Recombinant antibody (84298-1-RR)

    IF Staining of HepG2 using 84298-1-RR (same clone as 84298-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POTEA-Specific antibody (84298-1-RR, Clone: 241187F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84298-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POTEA-Specific Recombinant antibody (84298-1-RR)

    IF Staining of HeLa using 84298-1-RR (same clone as 84298-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using POTEA-Specific antibody (84298-1-RR, Clone: 241187F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84298-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using POTEA-Specific Recombinant antibody (84298-1-RR)

    FC experiment of HeLa using 84298-1-RR (same clone as 84298-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug POTEA-Specific Recombinant antibody (84298-1-RR, Clone:241187F7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84298-1-PBS in a different storage buffer formulation.

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POTEA-Specific Recombinant antibody, PBS Only

POTEA-Specific Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84298-1-PBS
Clone No.241187F7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

POTEA, POTE8, POTE 8, ANKRD26-like family A member 1, ANKRD26 like family A member 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84298-1-PBS targets POTEA-Specific in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name POTE ankyrin domain family, member A
Calculated molecular weight 56 kDa
GenBank accession numberNM_001002920
Gene Symbol POTEA
Gene ID (NCBI) 340441
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ6S8J7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

POTEA, also named A26A1 and POTE8, belongs to the POTE family. POTE has an important signaling function in the reproductive system.