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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POU2AF1 Polyclonal antibody (13605-1-AP)

    WB analysis using 13605-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 13605-1-AP (POU2AF1 antibody) at dilution of 1:5000 and incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using POU2AF1 Polyclonal antibody (13605-1-AP)

    WB analysis of mouse lung using 13605-1-AP

    mouse lung tissue was subjected to SDS PAGE followed by western blot with 13605-1-AP (POU2AF1 antibody) at dilution of 1:500 and incubated at room temperature for 1.5 hours.

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POU2AF1 Polyclonal antibody

POU2AF1 Polyclonal Antibody for WB, ELISA
Cat No. 13605-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

BOB 1, BOB1, OBF 1, OBF1, OCA B, OCAB, OCT binding factor 1, POU2AF1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, mouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13605-1-AP targets POU2AF1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POU2AF1 fusion protein Ag4482 相同性解析による交差性が予測される生物種
Full Name POU class 2 associating factor 1
Calculated molecular weight 256 aa, 27 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC032549
Gene Symbol POU2AF1
Gene ID (NCBI) 5450
ENSEMBL Gene IDENSG00000110777
RRIDAB_2252633
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16633
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.

Protocols

Product Specific Protocols
WB protocol for POU2AF1 antibody 13605-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols