OCT2 Polyclonal antibody
OCT2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 10867-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, mouse ovary tissue |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
10867-2-AP targets OCT2 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | OCT2 fusion protein Ag0955 相同性解析による交差性が予測される生物種 |
| Full Name | POU class 2 homeobox 2 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC006101 |
| Gene symbol | POU2F2 |
| Gene ID (NCBI) | 5452 |
| RRID | AB_2167080 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
POU2F2, also named as OCT2, is a 479 amino acid protein, which belongs to the POU transcription factor family. The Pit-Oct-Unc (POU) family of transcription factors includes many proteins that have been grouped in seven distinct classes according to function and expression containing proteins such as the neuronally expressed Pit proteins in class I, the ubiquitously expressed Oct-1 and tissue-specific Oct-2 in the second category, the well-known pluripotency marker Oct-4 which belongs to class V. Oct-1 (POU2f1) is a ubiquitous factor present in a wide range of cell types while Oct-2 (POU2f2) expression has been found confined to B lymphocytes and neuronal cells. Oct-2 has been implicated in B cell development and regulation of B lymphocyte-specific genes. POU2F2 exists various isoforms with MV 43, 50, and 62 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OCT2 antibody 10867-2-AP | Download protocol |
| IHC protocol for OCT2 antibody 10867-2-AP | Download protocol |
| IP protocol for OCT2 antibody 10867-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
