Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue, human placenta tissue, human skin tissue, human spleen tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue, human gliomas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 4 publications below |
Product Information
14596-1-AP targets BRN2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BRN2 fusion protein Ag6131 相同性解析による交差性が予測される生物種 |
| Full Name | POU class 3 homeobox 2 |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC051699 |
| Gene Symbol | BRN2 |
| Gene ID (NCBI) | 5454 |
| RRID | AB_2167371 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20265 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. It shares a highly homologous region that referred to as the POU domain. POU3F2 is a Class III POU genes that expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression. POU3F2 is required for maintaining neural cell differetiation and has a role in the production and positioning of neocortical neurons.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BRN2 antibody 14596-1-AP | Download protocol |
| IHC protocol for BRN2 antibody 14596-1-AP | Download protocol |
| IF protocol for BRN2 antibody 14596-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Glutathione S-transferase Pi (Gstp) proteins regulate neuritogenesis in the developing cerebral cortex. | ||
FEBS Lett miR-221/222 suppression induced by activation of the cAMP/PKA/CREB1 pathway is required for cAMP-induced bidirectional differentiation of glioma cells. | ||
Neurosci Lett Overexpression of the 14-3-3gamma protein in embryonic mice results in neuronal migration delay in the developing cerebral cortex. | ||
Neuron HDAC1 and HDAC2 Regulate Intermediate Progenitor Positioning to Safeguard Neocortical Development. | ||
Adv Sci (Weinh) Enabling Survival of Transplanted Neural Precursor Cells in the Ischemic Brain |