Validation Data Gallery

Filter: AllWBIHCFC (Intra)

WB analysis using 18998-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18998-1-AP (BRN2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18998-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18998-1-AP (BRN2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 18998-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18998-1-AP (BRN2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 18998-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 18998-1-AP (BRN2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 18998-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18998-1-AP (BRN2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18998-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18998-1-AP (BRN2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HEK-293 using 18998-1-AP
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human BRN2-Specific (18998-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

BRN2-Specific Polyclonal antibody

BRN2-Specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 18998-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), ELISA

Synonyms

BRN2, POU3F2, Brn-2, Brn 2, Brain-specific homeobox/POU domain protein 2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A375 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 1 publications below

Product Information

18998-1-AP targets BRN2-Specific in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	POU class 3 homeobox 2
Calculated molecular weight	47 kDa
Observed molecular weight	47-54 kDa
GenBank accession number	NM_005604
Gene Symbol	BRN2
Gene ID (NCBI)	5454
RRID	AB_10597389
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P20265
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. It shares a highly homologous region that referred to as the POU domain. POU3F2 is a Class III POU genes that expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression. POU3F2 is required for maintaining neural cell differetiation and has a role in the production and positioning of neocortical neurons.

Protocols

Product Specific Protocols
WB protocol for BRN2-Specific antibody 18998-1-AP	Download protocol
IHC protocol for BRN2-Specific antibody 18998-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Genes Dev Protein neddylation as a therapeutic target in pulmonary and extrapulmonary small cell carcinomas. Authors - Justin P Norton View Article
rat	WB	CNS Neurosci Ther Reactive astrocytes targeting with oral vitamin A: Efficient neuronal regeneration for Parkinson's disease treatment and reversal of associated liver fibrosis Authors - Nesrine Saeid El-Mezayen View Article
	IF	Elife Human-specific ARHGAP11B induces hallmarks of neocortical expansion in developing ferret neocortex. Authors - Nereo Kalebic View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 18998-1-AP

WB analysis of HeLa using 18998-1-AP

WB analysis of HEK-293 using 18998-1-AP

WB analysis of A375 using 18998-1-AP

IHC staining of mouse brain using 18998-1-AP