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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using POU3F4 Polyclonal antibody (25114-1-AP)

    WB analysis of fetal human brain using 25114-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25114-1-AP (POU3F4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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POU3F4 Polyclonal antibody

POU3F4 Polyclonal Antibody for WB, ELISA
Cat No. 25114-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Synonyms

BRAIN 4, Brn 4, BRN4, DFN3, DFNX2, Oct 9, Octamer binding protein 9, OTF 9, OTF9, POU class 3 homeobox 4, POU3F4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected infetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

25114-1-AP targets POU3F4 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POU3F4 fusion protein Ag18925 相同性解析による交差性が予測される生物種
Full Name POU class 3 homeobox 4
Calculated molecular weight 361 aa, 39 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC146551
Gene Symbol POU3F4
Gene ID (NCBI) 5456
RRIDAB_2879903
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49335
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OTF9 also named as BRN4 and POU3F4, belongs to the POU transcription factor family and Class-3 subfamily. OTF9 is a transcription factor which exerts its primary action widely during early neural development and in a very limited set of neurons in the mature brain. Mutation of OTF9 will cause of X-linked deafness type 3 (DFN3).

Protocols

Product Specific Protocols
WB protocol for POU3F4 antibody 25114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Biol Eng

An engineered ligand-responsive Csy4 endoribonuclease controls transgene expression from Sendai virus vectors

Authors - Takumi Kishimoto
View Article