POU4F2 Polyclonal antibody
POU4F2 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 55042-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | L02 cells, mouse eye tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
55042-1-AP targets POU4F2 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | POU class 4 homeobox 2 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 40-44 kDa |
| GenBank accession number | NM_004575 |
| Gene symbol | POU4F2 |
| Gene ID (NCBI) | 5458 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
POU4F2,also named as BRN3.2, BRN3B and Brn-3b, belongs to the POU transcription factor family and Class-4 subfamily. It is a transcription factor. POU4F2 may play a role in determining or maintaining the identities of a small subset of visual system neurons. The antibody is specific to POU4F2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for POU4F2 antibody 55042-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Stem Cell Res Ther Protective effects of human iPS-derived retinal pigmented epithelial cells on retinal degenerative disease. | ||
Sci Rep 661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively. | ||
Neurochem Int Human primary retinal cells as an in-vitro model for investigating defective signalling caused by OPTN mutants associated with glaucoma. | ||
Life Sci Intervention of epithelial mesenchymal transition against colon cancer cell growth and metastasis based on SOX21/POU4F2/Hedgehog signaling axis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Alessandro (Verified Customer) (12-09-2023) | good Ab for retinal ganglio cells
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