Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of human heart using 14985-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 14985-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 14985-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14985-1-AP (PPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 14985-1-AP
IP result of anti-PPA1 (IP:14985-1-AP, 4ug; Detection:14985-1-AP 1:1000) with mouse liver tissue lysate 6000 ug.
IHC staining of human breast cancer using 14985-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14985-1-AP (PPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14985-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14985-1-AP (PPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 14985-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14985-1-AP (PPA1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 14985-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14985-1-AP (PPA1 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 14985-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14985-1-AP (PPA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PPA1 Polyclonal antibody

PPA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14985-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PPase, PP1, PP, IOPPP, Inorganic pyrophosphatase

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human heart tissue, human liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human breast cancer tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

14985-1-AP targets PPA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PPA1 fusion protein Ag6969 相同性解析による交差性が予測される生物種
Full Name	pyrophosphatase (inorganic) 1
Calculated molecular weight	33 kDa
Observed molecular weight	33 kDa
GenBank accession number	BC001022
Gene Symbol	PPA1
Gene ID (NCBI)	5464
RRID	AB_2167890
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15181
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).

Protocols

Product Specific Protocols
WB protocol for PPA1 antibody 14985-1-AP	Download protocol
IHC protocol for PPA1 antibody 14985-1-AP	Download protocol
IF protocol for PPA1 antibody 14985-1-AP	Download protocol
IP protocol for PPA1 antibody 14985-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Metabolites PPA1 Deficiency Causes a Deranged Galactose Metabolism Recognizable in Neonatal Screening Authors - Melanie T Achleitner View Article
human	WB	Sci Rep Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology. Authors - Hexiao Shen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human heart using 14985-1-AP

WB analysis of human liver using 14985-1-AP

WB analysis of human liver using 14985-1-AP

IP experiment of mouse liver using 14985-1-AP

IHC staining of human breast cancer using 14985-1-AP