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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPA1 Monoclonal antibody (68178-1-Ig)

    WB analysis using 68178-1-Ig (same clone as 68178-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68178-1-Ig (PPA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68178-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPA1 Monoclonal antibody (68178-1-Ig)

    IF Staining of HeLa using 68178-1-Ig (same clone as 68178-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PPA1 antibody (68178-1-Ig, Clone: 1D11F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68178-1-PBS in a different storage buffer formulation.

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PPA1 Monoclonal antibody, PBS Only

PPA1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68178-1-PBS
Clone No.1D11F4

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Inorganic pyrophosphatase, IOPPP, PP, PP1, PPA1, PPase, pyrophosphatase (inorganic) 1, SID6 8061

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68178-1-PBS targets PPA1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPA1 fusion protein Ag7049 相同性解析による交差性が予測される生物種
Full Name pyrophosphatase (inorganic) 1
Calculated molecular weight 33 kDa
Observed molecular weight33 kDa
GenBank accession numberBC001022
Gene Symbol PPA1
Gene ID (NCBI) 5464
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15181
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).