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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPA2 Polyclonal antibody

PPA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16662-1-AP

Synonyms

HSPC124, PPA2, PPase 2, pyrophosphatase (inorganic) 2, Pyrophosphatase SID6 306, SID6 306

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using PPA2 Polyclonal antibody (16662-1-AP)

    WB analysis of mouse heart using 16662-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16662-1-AP (PPA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PPA2 Polyclonal antibody (16662-1-AP)

    WB analysis using 16662-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16662-1-AP (PPA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using PPA2 Polyclonal antibody (16662-1-AP)

    WB analysis of mouse kidney using 16662-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16662-1-AP (PPA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PPA2 Polyclonal antibody (16662-1-AP)

    IHC staining of human liver cancer using 16662-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PPA2 Polyclonal antibody (16662-1-AP)

    IHC staining of human liver cancer using 16662-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PPA2 Polyclonal antibody (16662-1-AP)

    IHC staining of human liver cancer using 16662-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PPA2 Polyclonal antibody (16662-1-AP)

    IHC staining of human liver cancer using 16662-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PPA2 Polyclonal antibody (16662-1-AP)

    IHC staining of human lung cancer using 16662-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PPA2 Polyclonal antibody (16662-1-AP)

    IHC staining of human lung cancer using 16662-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse heart tissue, mouse kidney tissue, mouse liver tissue, rat heart tissue
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

16662-1-AP targets PPA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPA2 fusion protein Ag10138 相同性解析による交差性が予測される生物種
Full Name pyrophosphatase (inorganic) 2
Calculated molecular weight305aa,35 kDa; 334aa,38 kDa
Observed molecular weight 32-40 kDa
GenBank accession numberBC057219
Gene symbol PPA2
Gene ID (NCBI) 27068
RRIDAB_1851549
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pyrophosphatase 2 (PPA2) is a mitochondrial-located pyrophosphatase (PPase) that hydrolyzes inorganic pyrophosphate (PPi), generated by numerous nucleotide-dependent reactions, into two orthophosphate molecules (PMID: 34400813). PPA2 is a cardiomyopathy-associated protein, which has a greater physiological importance in mitochondrial function (PMID: 27523597).

Protocols

Product Specific Protocols
WB protocol for PPA2 antibody 16662-1-APDownload protocol
IHC protocol for PPA2 antibody 16662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Immunol

Multi-omics pan-cancer analysis reveals the prognostic values and immunological functions of PPA2, with a spotlight on breast cancer

Authors - Jia-Ning Zhang
View Article